Hi, I encountered something strange the other day with my western blot: after transfer, the pre-stained ladder (bio-rad) showed up fine, but there was no protein whatsoever on the blot stained with Ponceau. Not intended for human or animal diagnostic or therapeutic uses. Ladder but no protein - (Aug/24/2009 ) Ladder but no protein. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Comparison chart and selection guide for protein gel electrophoresis size standards, ladders, and molecular weight markers (MW markers), including unstained and prestained varieties for in-gel or western blot detection. Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10250 kD). 2.5 μl per well for general Western transferring.Ģ0 mM Tris-phosphate(pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. The ladder is supplied in the gel loading buffer and is ready to use.ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa.
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